Peptostreptococcus elsdenii from the caecum of pigs.

Identification and properties of new flavins in electron-transferring flavoprotein from Peptostreptococcus elsdenii and pig-liver glycolate oxidase.

I. New flavins have been isolated from purified preparations of an electron-transferring flavoprotein (ETF) from Peptostreptococcus elsdenii and glycolate oxidase from pig liver. The structures of these new species have been established as FAD and FMN derivatives of 6-hydroxy-7,8-dimethyl-isoalloxazine, the chemical synthesis of which is described in the accompanying paper by Schollnhammer and ...

Electron-nuclear double resonance study of flavodoxin from Peptostreptococcus elsdenii.

Dependence of the proton magnetic resonance spectra on the oxidation state of flavodoxin from Clostridium MP and from Peptostreptococcus elsdenii.

The broadening of protein nuclear magnetic resonances in the spectra of the semiquinone forms of flavodoxins derived from Clostridium MP and Peptostreptococcus elsdenii relative to the resonances in the oxidized and reduced forms is highly selective. Spectra from both species of flavodoxin indicate that conformational differences between the oxidized and fully reduced states are minor and, cons...

Bba 66291 Studies on Flavin Binding in Flavodoxins

I. Stable apoproteins have been prepared from Peptostreptococcus elsdenii, C. pasteurianum and Clostridium MP flavodoxins by dialysis of the native proteins against 2 M KBr at pH 3.9 and 3" lO-4 M EDTA. The apoproteins each bind I molecule of FMN to give complexes identical with the native flavodoxins. 2. Binding causes almost complete quenching of both protein and FMN fluorescence. This proper...

Importance of the isovalerate carboxylation pathway of leucine biosynthesis in the rumen.

Certain anaerobic ruminal bacteria synthesize the leucine carbon skeleton by use of a pathway different from that described in other microorganisms. These organisms carboxylate the intact carbon skeleton of isovalerate, synthesizing leucine-2-C(14) from isovalerate-1-C(14). Strains of Bacteroides ruminicola and Peptostreptococcus elsdenii were like Ruminococcus flavefaciens in that they incorpo...